Composite
BluetL-BS

Part:BBa_K4256666:Design

Designed by: KaMing Li   Group: iGEM22_UCAS-China   (2022-10-09)


BluetL (Blue light & Low Temperature) Bistable-Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1620
    Illegal NheI site found at 1733
    Illegal NheI site found at 1756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2418
    Illegal BamHI site found at 111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To verify the feasibility of the switch, The UCAS-China iGEM team 2022 designed two plasmids (pET-28a) containing our switch sequences. With four different fluorescent proteins added as reporters, we can quantify the feasibility of our design. In the absence of blue light, YFP (yellow) and EYFP (cyan) proteins will be produced at 37°C and 25°C respectively. In contrast upon blue light excitation, the reporter located at the other side of the switch will separately fabricate mCherry (red) and JGFP (green) proteins at 37°C and 25°C.

Source

BBa_K3247005 BBa_K2469004

References